Cellular responses on anodized titanium discs coated with 1α,25-dihydroxyvitamin D3 incorporated Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles
조영진, 허성주, 곽재영, 김성균, 이주희,
소속 상세정보
조영진 ( Cho Young-Jin ) - 서울대학교 치과대학 치과보철학교실
허성주 ( Heo Seong-Joo ) - 서울대학교 치과대학 치과보철학교실
곽재영 ( Koak Jai-Young ) - 서울대학교 치과대학 치과보철학교실
김성균 ( Kim Seong-Kyun ) - 서울대학교 치과대학 치과보철학교실
이주희 ( Lee Joo-Hee ) - 울산대학교 의과대학 서울아산병원 치과학교실
KMID : 0362420080460060620
Abstract
Statement of Problem: A biochemical approach for surface modification has offered an alternative for physicochemical and morphological methods to obtain desirable bone-implant interfaces.
Purpose: The purpose of the present study was to investigate cell responses to poly (D,L-lactide-co-glycolide) (PLGA)/1α,25-(OH)2D3 coating with reference to cellular proliferation and differentiation in vitro.
Material and Methods: 96 titanium discs were fabricated and divided into four groups. Group 1 was anodized under 300 V as control. Group 2, 3 and 4 were anodized then coated with 3 ml PLGA/1α,25-(OH)2D3 solutions. Amount of the solutions were 2 ul, 20 ul and 200ul respectively. The osteoblast-like Human Osteogenic Sarcoma (HOS) cells were seeded and cultured for 1, 3 and 7 days. MTSbased cell proliferation assay and ALPase activity test were carried out.
Results: PLGA nanoparticles were observed as fine, smooth and round and HOS cells attached to the anodized surfaces through strand-like and sheet-like filopodia. After 3 days of culture, the dendritic filopodia were exaggerated and sheet-like cytoplasmic projections covered the coated titanium surfaces. After 3 days of culture, all of the groups showed increased cellular proliferation and the lowest proliferation rate was measured on group 2. Higher amount of incorporated 1 α,25-(OH)2D3 (Group 3 and 4) improved cellular proliferation but the differences were not significant statistically (P > .05). But they increased the rate of ALP activities than the control group at day 3 (P < .05).
Conclusion: Biodegradable PLGA nanoparticles incorporated with vitamin D metabolite positively affected proliferation and differentiation of cells on the anodized titanium surface.
키워드
1α;25-dihydroxyvitamin D3;PLGA;Nanoparticle;Anodized titanium;Cell proliferation;Differentiation
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